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Design of a comprehensive biochemistry and molecular biology experiment: Phase variation caused by recombinational regulation of bacterial gene expression
Author(s) -
Sheng Xiumei,
Xu Shungao,
Lu Renyun,
Isaac Dadzie,
Zhang Xueyi,
Zhang Haifang,
Wang Huifang,
Qiao Zheng,
Huang Xinxiang
Publication year - 2014
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.20772
Subject(s) - phase variation , biology , direct agglutination test , gene , experimental biology , salmonella , computational biology , microbiology and biotechnology , biochemistry , genetics , antibody , bacteria , serology , phenotype
Scientific experiments are indispensable parts of Biochemistry and Molecular Biology. In this study, a comprehensive Biochemistry and Molecular Biology experiment about Salmonella enterica serovar Typhi Flagellar phase variation has been designed. It consisted of three parts, namely, inducement of bacterial Flagellar phase variation, antibody agglutination test, and PCR analysis. Phase variation was observed by baterial motility assay and identified by antibody agglutination test and PCR analysis. This comprehensive experiment can be performed to help students improve their ability to use the knowledge acquired in Biochemistry and Molecular Biology. © 2014 by The International Union of Biochemistry and Molecular Biology, 42(3):–, 2014.