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Two‐dimensional nuclear magnetic resonance structure determination module for introductory biochemistry: synthesis and structural characterization of lyso‐glycerophospholipids
Author(s) -
Garrett Teresa A.,
Rose Rebecca L.,
Bell Sidney M.
Publication year - 2013
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.20668
Subject(s) - glycerophospholipids , lyso , characterization (materials science) , chemistry , biochemistry , stereochemistry , computer science , nanotechnology , materials science , scintillator , phospholipid , telecommunications , membrane , detector
In this laboratory module, introductory biochemistry students are exposed to two‐dimensional 1 H‐nuclear magnetic resonance of glycerophospholipids (GPLs). Working in groups of three, students enzymatically synthesized and purified a variety of 2‐acyl lyso GPLs. The structure of the 2‐acyl lyso GPL was verified using 1 H‐correlation spectroscopy. Students scored significantly higher on an assessment of NMR knowledge after having participated in this lab module and in comparison to a similar cohort who did not participate. Inaddition, student confidence in their NMR knowledge and abilities increased 62% following the module and correlated with their ability to apply their NMR knowledge. Based on these results, the laboratory module was very effective at providing students with a more extensive understanding of the underlying concepts of NMR as a tool for structural determination. © 2013 by The International Union of Biochemistry and Molecular Biology 41(2):87‐94, 2013