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Problem‐solving test: Nucleocytoplasmic shuttling of pre‐mrna binding proteins
Author(s) -
Szeberényi‡ József
Publication year - 2012
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.20652
Subject(s) - rna , ribonuclease , microbiology and biotechnology , gel electrophoresis , messenger rna , chemistry , polyacrylamide gel electrophoresis , blot , ribosomal rna , rna polymerase ii , transcription (linguistics) , chromatography , biology , biochemistry , gene expression , enzyme , gene , linguistics , promoter , philosophy
Terms to be familiar with before you start to solve the test: transcription, pre‐mRNA, RNA processing, RNA transport, RNA polymerase II, direct and indirect immunofluorescence staining, cell fractionation by centrifugation, oligo(dT)‐cellulose chromatography, washing and elution of the column, ribonuclease, SDS‐polyacrylamide gel electrophoresis, Western‐blotting, pre‐ribosomal particles, nuclear envelope, heterokaryon, phase contrast microscopy, polyadenylated RNA.