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The use of biotin to demonstrate immunohistochemistry, western blotting, and dot blots in university practical classes
Author(s) -
Millar Thomas James,
Knighton Ronald,
Chuck JoAnne
Publication year - 2012
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.20607
Subject(s) - biotinylation , blot , biotin , task (project management) , class (philosophy) , resource (disambiguation) , computer science , immunohistochemistry , biology , computational biology , microbiology and biotechnology , biochemistry , immunology , artificial intelligence , engineering , systems engineering , computer network , gene
Immunological detection of proteins is an essential method to demonstrate to undergraduate biology students, however, is often difficult in resource and time poor student laboratory sessions. This method describes a failsafe method to rapidly and economically demonstrate this technique using biotinylated proteins or biotin itself as targets for detection. This negates the need for a specific primary antibody, saving cost and time. In addition, the easily available and safe reagents allow the methods to be readily adopted without specialist technical expertise. As a result, staff can confidently transfer ownership of the task to the student so as to also develop scientific inquiry skills which promotes student motivation and engagement.