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Using Pseudomonas putida xylE gene to teach molecular cloning techniques for undergraduates
Author(s) -
Dong Xu,
Xin Yi,
Ye Li,
Ma Yufang
Publication year - 2009
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.20328
Subject(s) - pseudomonas putida , cloning (programming) , molecular cloning , catechol , computational biology , biology , gene , microbiology and biotechnology , genetics , biochemistry , gene expression , computer science , programming language
We have developed and implemented a serial experiment in molecular cloning laboratory course for undergraduate students majored in biotechnology. Pseudomonas putida xylE gene, encoding catechol 2, 3‐dioxygenase, was manipulated to learn molecular biology techniques. The integration of cloning, expression, and enzyme assay gave students a chance to mimic real research and product development by connecting separated techniques. This serial experiment helped students set up intact idea of molecular biology and to understand the relation of each individual operation.