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RNase one gene isolation, expression, and affinity purification models research experimental progression and culminates with guided inquiry‐based experiments
Author(s) -
Bailey Cheryl P.
Publication year - 2009
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.20247
Subject(s) - context (archaeology) , computational biology , isolation (microbiology) , gene , session (web analytics) , rnase p , microbiology and biotechnology , biochemistry , chemistry , biology , computer science , bioinformatics , world wide web , rna , paleontology
This new biochemistry laboratory course moves through a progression of experiments that generates a platform for guided inquiry‐based experiments. RNase One gene is isolated from prokaryotic genomic DNA, expressed as a tagged protein, affinity purified, and tested for activity and substrate specificity. Student pairs present detailed explanations of materials and methods and the semester culminates in a poster session. Experimental plans take into account the expense and time required to move from gene isolation to enzyme assays. This combination of instructor‐guided and student‐designed experiments is a manageable foray into guided inquiry‐based learning in a biochemistry laboratory course, while providing a cohesive story and context for individual experiments.