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Interrelationship between oxidative damage and antioxidant enzyme activities: An easy and rapid experimental approach
Author(s) -
Matés J. M.,
Aledo J. C.,
PérezGómez C.,
del Valle A. Esteban,
Segura J. M.
Publication year - 2008
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.20241
Subject(s) - antioxidant , oxidative damage , oxidative phosphorylation , volume (thermodynamics) , biochemistry , humanities , chemistry , philosophy , physics , thermodynamics
This article describes a method for determining some antioxidant enzyme activities (catalase and/or glutathione peroxidase) and the oxidative status (protein oxidative damage and/or lipid peroxidation) of human blood. However, the main objective of the work is to illustrate the relationship between antioxidant defences and oxidative damage, showing to students their correlation and the general importance of the biochemical regulation in health and diseases.