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Analyzing ligand depletion in a saturation equilibrium binding experiment
Author(s) -
Claro Enrique
Publication year - 2006
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.2006.494034062677
Subject(s) - muscarinic acetylcholine receptor , saturation (graph theory) , ligand (biochemistry) , quinuclidinyl benzilate , chemistry , membrane , receptor , biophysics , biology , biochemistry , mathematics , combinatorics
I present a proposal for a laboratory practice to generate and analyze data from a saturation equilibrium binding experiment addressed to advanced undergraduate students. [ 3 H]Quinuclidinyl benzilate is a nonselective muscarinic ligand with very high affinity and very low nonspecific binding to brain membranes, which contain a high density of muscarinic receptors. These features allow the instructor to devote especial emphasis to evaluate ligand depletion, and therefore, stress the subtle but fundamental difference between total (added) ligand and free ligand concentration at equilibrium.