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Use of solid phase extraction in the biochemistry laboratory to separate different lipids
Author(s) -
Flurkey William H.
Publication year - 2005
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.2005.49403305357
Subject(s) - chromatography , solid phase extraction , phospholipid , chemistry , extraction (chemistry) , diacylglycerol kinase , silica gel , cartridge , fatty acid , biochemistry , enzyme , materials science , membrane , protein kinase c , metallurgy
Solid‐phase extraction (SPE) was used to demonstrate how various lipids and lipid classes could be separated in a biochemistry laboratory setting. Three different SPE methods were chosen on their ability to separate a lipid mixture, consisting of a combination of a either a fatty acid, a triacylglycerol, a mono‐ or diacylglycerol, phospholipid, cholesterol, or cholesteryl ester into distinct lipid fractions. These mini‐scale SPE methods used aminopropyl‐bonded silica columns or silica Sep‐Pak cartridges suitable for completion in a 2‐ to 3‐h time block. Thin‐layer chromatography of the isolated lipid fractions was used to show separation characteristics and to evaluate the utility and reproducibility of each SPE method.