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Determination of the Rh factor: A practical illustrating the use of the polymerase chain reaction
Author(s) -
Imperial Santiago,
Boronat Albert
Publication year - 2005
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.2005.494033010425
Subject(s) - polymerase chain reaction , session (web analytics) , template , computational biology , dna , microbiology and biotechnology , gel electrophoresis , chemistry , chromatography , biology , biochemistry , computer science , nanotechnology , gene , materials science , world wide web
A practical experiment on the PCR is described that has been used over several years as part of an undergraduate biochemistry and molecular biology course for chemistry students. In the first experimental session, students prepare their own DNA samples from epithelial cells of the mouth and use them as templates in the PCR. In the second session, they analyze the amplified DNA by electrophoresis and determine their Rh factor.