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Development of a green fluorescent protein‐based laboratory curriculum
Author(s) -
Larkin Patrick D.,
Hartberg Yasha
Publication year - 2005
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.2005.494033010417
Subject(s) - green fluorescent protein , plasmid , recombinant dna , transformation (genetics) , biology , dna , computational biology , microbiology and biotechnology , dna sequencing , biochemistry , gene
A laboratory curriculum has been designed for an undergraduate biochemistry course that focuses on the investigation of the green fluorescent protein (GFP). The sequence of procedures extends from analysis of the DNA sequence through PCR amplification, recombinant plasmid DNA synthesis, bacterial transformation, expression, isolation, and characterization of the protein by SDS‐PAGE. A survey of participants found that the majority of them were performing most of these procedures for the first time and that participants found the exercises enjoyable and considered them a significant aid to their understanding of biochemistry, cell biology, and molecular genetics.