Premium
Immunoassay for visualization of protein‐protein interactions on Ni‐nitrilotriacetate support: Example of a laboratory exercise with recombinant heterotrimeric Gα i2 β 1γ2 Tagged by Hexahistidine from sf9 Cells
Author(s) -
Bavec Aljosa
Publication year - 2004
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.2004.494032040380
Subject(s) - heterotrimeric g protein , sf9 , chemistry , recombinant dna , protein subunit , visualization , g protein , biochemistry , computer science , data mining , gene , receptor , spodoptera
We have developed an in vitro assay for following the interaction between the α i2 subunit and β 1γ2 dimer from sf9 cells. This method is suitable for education purposes because it is easy, reliable, nonexpensive, can be applied for a big class of 20 students, and avoid the commonly used kinetic approach, which does not allow visualization of protein‐protein interaction.