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Formation and detoxification of reactive oxygen species
Author(s) -
Kuciel Radosława,
Mazurkiewicz Aleksandra
Publication year - 2004
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.2004.494032030352
Subject(s) - xanthine oxidase , superoxide , allopurinol , catalase , superoxide dismutase , reactive oxygen species , chemistry , detoxification (alternative medicine) , oxygen , xanthine , biochemistry , oxidase test , antioxidant , enzyme , organic chemistry , medicine , alternative medicine , pathology
A model of reactive oxygen species metabolism is proposed as a laboratory exercise for students. The superoxide ion in this model is generated during the reaction of oxidation of xanthine, catalyzed by xanthine oxidase. The effect of catalase, superoxide dismutase, and allopurinol on superoxide ion generation and removal in this system is also determined, using cytochrome c as a probe.