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The zymogen‐enteropeptidase system: A practical approach to study the regulation of enzyme activity by proteolytic cleavage *
Author(s) -
Pizauro João M.,
Ferro Jesus A.,
de Lima Andréa C. F.,
Routman Karina S.,
Portella Maria Célia
Publication year - 2004
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.2004.494032010311
Subject(s) - zymogen , trypsinogen , enteropeptidase , chymotrypsinogen , proteolytic enzymes , chemistry , enzyme , cleavage (geology) , biochemistry , trypsin , biology , chymotrypsin , paleontology , fracture (geology) , fusion protein , gene , recombinant dna
The present research describes an efficient procedure to obtain high levels of trypsinogen and chymotrypsinogen by using a simple, rapid, and easily reproducible method. The extraction process and the time‐course of activation of zymogens can be carried out in a single laboratory period, without sophisticated equipment. The main objective was to prepare a laboratory class that would stimulate student interest in enzyme regulation, exploring the fact that the catalytic activity of some enzymes is regulated by different mechanisms. The regulation of proteolytic enzymes requires the synthesis of an inactive zymogen and its being irreversibly “switched on” by specific proteolytic cleavage.