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A spectroscopic‐based laboratory experiment for protein conformational studies *
Author(s) -
Ramos Carlos Henrique I.
Publication year - 2004
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.2004.494032010309
Subject(s) - circular dichroism , conformational change , absorbance , structural biology , biophysics , chemistry , protein structure , biochemistry , chromatography , biology
This article describes a practical experiment for teaching basic spectroscopic techniques to introduce the topic of protein conformational change to students in the field of molecular biology, biochemistry, or structural biology. The spectroscopic methods employed in the experiment are absorbance, for protein concentration measurements, and fluorescence and circular dichroism, for probing protein conformational changes.