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DNA isolation from a dried blood sample, PCR amplification, and population analysis: Making the most of commercially available kits
Author(s) -
DeLong Frost Laura,
Peart Sharifa T.
Publication year - 2003
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.2003.494031060268
Subject(s) - dna extraction , biology , population , polymerase chain reaction , microbiology and biotechnology , computational biology , dna , isolation (microbiology) , genetics , bioinformatics , gene , medicine , environmental health
A 3‐week undergraduate molecular biology laboratory exercise combining commercially available kits is described. The laboratory involves DNA extraction from a human dried blood sample, amplification of an Alu‐repeat sequence found on chromosome 8, and analysis of the genetic and allelic frequency in the class population using Hardy‐Weinberg statistics. The use of the prepared kits provides a means for the instructor of an undergraduate biochemistry course to explore molecular biology techniques on a limited budget and with a minimal time investment.