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Transcriptional and posttranslational regulation of a membrane nutrient transporter
Author(s) -
Stambuk Boris U.
Publication year - 2002
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.2002.494030060136
Subject(s) - maltose , permease , transporter , glucose transporter , biochemistry , yeast , green fluorescent protein , endocytosis , intracellular , membrane transport protein , chemistry , saccharomyces cerevisiae , biology , enzyme , gene , cell , insulin , endocrinology
A laboratory exercise illustrating the metabolic regulation of a plasma membrane transporter was devised. A simple colorimetric assay allows the determination of the yeast maltose permease activity under different conditions, and thus the induction of the transporter by maltose and its repression by glucose can be easily demonstrated. The laboratory exercise also shows that glucose regulates the expression of the transporter at the plasma membrane by promoting its inactivation and endocytosis. The intracellular trafficking of the transporter under these conditions can be further illustrated using a maltose permease tagged with the green fluorescent protein. The abbreviations used are: 2‐dGlu, 2‐deoxy‐ D ‐glucose, GFP, green fluorescent protein; p NPαG, p ‐nitrophenyl‐α‐ D ‐glucopyranoside; YNB, yeast nitrogen base without amino acids and ammonium sulfate.