Premium
Promotion of photodynamic therapy‐induced death: Laboratory experimental methodology
Author(s) -
Vittar Natalia Belén Rumie,
Galetto Román,
Yslas Edith Inés,
Durantini Edgardo Néstor,
Rivarola Viviana
Publication year - 2002
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.2002.494030050118
Subject(s) - photodynamic therapy , photosensitizer , apoptosis , programmed cell death , promotion (chess) , necrosis , viability assay , cancer research , medicine , chemistry , pathology , biochemistry , political science , organic chemistry , politics , law
Photodynamic therapy, a cancer treatment using a photosensitizer and visible light, has been shown to induce apoptosis or necrosis. In the present paper we recommend the use of a phthalocyanine derivative in an experimental class with the purpose of introducing students to photodynamic treatment of cultured cells, morphological observation of cell death, and viability assays. We consider that this activity might help students trained previously in tissue culture handling to experience a richer way of learning concepts and techniques.