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The free energy of denaturation of lysozyme: An undergraduate experiment in biophysical chemistry *
Author(s) -
Kurtin William E.,
Lee John M.
Publication year - 2002
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.2002.494030040083
Subject(s) - lysozyme , denaturation (fissile materials) , guanidine , chemistry , urea , hydrochloride , biophysical chemistry , chromatography , biophysics , biochemistry , nuclear chemistry , biology
An undergraduate laboratory experiment to measure the free energy of denaturation of the enzyme lysozyme is described. The fluorescence of the protein in solutions of varying concentrations of urea and guanidine hydrochloride is measured. The data are analyzed graphically assuming a two‐state model for the denaturation.