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Liposomes as tools to study drug diffusion and toxin‐induced leaks
Author(s) -
Levillain Florence,
Lanéelle Gilbert
Publication year - 2002
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.2002.494030020052
Subject(s) - phosphatidic acid , liposome , vesicle , chemistry , phospholipid , chromatography , phosphatidylcholine , membrane , biophysics , biochemistry , biology
Multilayered vesicles made of egg‐phosphatidylcholine and of phosphatidic acid were used to teach in a 4‐h session of practical work with a low cost spectrophotometer how to determine osmolarity inside multilayered vesicles and to show, by using two anti‐tuberculous drugs (isonicotinic acid hydrazide, p ‐aminosalicylate), that a small and non‐ionized molecule diffused freely through phospholipid vesicles, whereas a charged one did not. In addition, the permeabilizing effect of melittin, a membrane‐targeted bee‐venom toxin, was tested.