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Detection of genetically modified organisms in food by DNA extraction and PCR amplification
Author(s) -
Thion Laurent,
Vossen Christine,
Couderc Bettina,
Erard Monique,
Clemençon Blandine
Publication year - 2002
Publication title -
biochemistry and molecular biology education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 39
eISSN - 1539-3429
pISSN - 1470-8175
DOI - 10.1002/bmb.2002.494030010037
Subject(s) - dna extraction , dna , genetically modified organism , polymerase chain reaction , biology , microbiology and biotechnology , extraction (chemistry) , chromatography , computational biology , biochemistry , chemistry , gene
A practical class experiment based on the extraction/purification of DNA from soybeans and the identification of exogenous DNA sequences amplified by PCR is described. This is used in a biochemistry and molecular biology course for advanced science undergraduate students. Students are given small amounts of soybean powder, and they practice a DNA extraction, purification, and a PCR with a set of different specific primers followed by an electrophoresis to determine whether the sample is transgenic. A major aim is to provide experience in the use of common techniques such as DNA extraction/purification and PCR and their interpretation.