Detection of genetically modified organisms in food by DNA extraction and PCR amplification | Zendy

Thion Laurent | Zendy; Vossen Christine | Zendy; Couderc Bettina | Zendy; Erard Monique | Zendy; Clemençon Blandine | Zendy

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Detection of genetically modified organisms in food by DNA extraction and PCR amplification

Author(s) -

Thion Laurent,

Vossen Christine,

Couderc Bettina,

Erard Monique,

Clemençon Blandine

Publication year - 2002

Publication title -

biochemistry and molecular biology education

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.34

H-Index - 39

eISSN - 1539-3429

pISSN - 1470-8175

DOI - 10.1002/bmb.2002.494030010037

Subject(s) - dna extraction , dna , genetically modified organism , polymerase chain reaction , biology , microbiology and biotechnology , extraction (chemistry) , chromatography , computational biology , biochemistry , chemistry , gene

A practical class experiment based on the extraction/purification of DNA from soybeans and the identification of exogenous DNA sequences amplified by PCR is described. This is used in a biochemistry and molecular biology course for advanced science undergraduate students. Students are given small amounts of soybean powder, and they practice a DNA extraction, purification, and a PCR with a set of different specific primers followed by an electrophoresis to determine whether the sample is transgenic. A major aim is to provide experience in the use of common techniques such as DNA extraction/purification and PCR and their interpretation.

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