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Integration of Surface‐enhanced Raman Spectroscopy with PCR for Monitoring Single Copy of KRAS G12D Mutation
Author(s) -
Sanchez Barea Joel,
Kang DongKu
Publication year - 2021
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.12298
Subject(s) - kras , mutation , computational biology , cancer research , polymerase chain reaction , biology , microbiology and biotechnology , genetics , gene
Identification and monitoring cancer‐related genetic mutation become a powerful tool for targeted therapy in recent decades and KRAS is one of the most frequently mutated oncogenes in many types of cancers. Polymerase chain reaction (PCR) is one of the most powerful diagnostic tools and it is widely used for monitoring infectious diseases and tumor‐related mutations. However, there are still limitations for monitoring rare mutations in complex biological samples and also for monitoring various mutations in a multiplexed manner from a single set assay. In this study, we present an integration of surface‐enhanced Raman scattering (SERS) with PCR that can be used for monitoring KRAS G12D mutation and also can distinguish from similar mutations such as KRAS G13D, G12S, and G12V. With this approach, various KRAS mutations can be detected from a single set of experiment without complex procedures or microdevices.