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A Dual‐Functional Lactate Sensor Based on Silver Nanoparticle‐coated Carbon Dots
Author(s) -
Park Joo Hee,
Yu Kai,
Min JinYoung,
Chung YoungHo,
Yoon Ji Young
Publication year - 2021
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.12257
Subject(s) - silver nanoparticle , x ray photoelectron spectroscopy , fluorescence , nanoparticle , detection limit , chemistry , nuclear chemistry , catalysis , selectivity , analytical chemistry (journal) , carbon fibers , photochemistry , materials science , nanotechnology , chemical engineering , chromatography , organic chemistry , physics , quantum mechanics , composite number , engineering , composite material
Herein, a silver nanoparticle‐coated carbon dots (Ag‐CDs) was fabricated to detect lactate using colorimetric and fluorometric methods. When the Ag‐CDs were decomposed by H 2 O 2 produced from the lactate oxidase (LOx) catalytic reaction, the color of the Ag‐CDs solution changed from yellowish to transparent, and the fluorescence of the quenched CDs was simultaneously recovered. Silver nanoparticles (Ag NPs) decomposition was confirmed by X‐ray photoelectron spectroscopy, revealing an increase in AgN bond peaks due to ionization of Ag NPs. The Ag‐CDs sensor could recognize lactate specifically, even in the presence of numerous potentially interfering biomarkers. The fabricated sensor displayed good sensitivity and selectivity, with a low detection limit of 0.6 μM and 10 μM for H 2 O 2 and lactate sensing, respectively. Finally, the Ag‐CDs sensor could be applied to determination of lactate to intracellular imaging in 4T1 cells using confocal microscopy.