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Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry‐based Structural Analysis of Deacylated Lipooligosaccharides From Escherichia coli
Author(s) -
Choi Yu Ri,
Kim Kwang Sung,
Bandu Raju,
Kim Hyoseon,
Lee Jae Eun,
Shin ByongKyu,
Cho Yang Je,
Park Jong Moon,
Lee Hookeun,
Kim Kwang Pyo
Publication year - 2020
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.11993
Subject(s) - chromatography , electrospray ionization , chemistry , tandem mass spectrometry , mass spectrometry , lipid a , electrospray , liquid chromatography–mass spectrometry , bacteria , biology , genetics
Deacylated lipooligosaccharides (dLOSs) were developed as human vaccine adjuvants. The dLOSs have significant immune activities and markedly lower toxicities than their precursors, lipopolysaccharides (LPSs). In this study, we developed a novel liquid chromatography mass spectrometry (LC–MS) method for characterization of the dLOSs. The structural identification and characterization of the dLOSs were carried out using electrospray ionization tandem mass spectrometric experiments. Finally, we propose the most likely structure of the dLOSs as being composed of a diacyl form of lipid A with seven sugars, 3 HEP + 2 KDO + 1 GlcN + 1 Glc.