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Base Pair Distance in Single‐DNA Molecule via TIRF‐Based Super‐Resolution Radial Fluctuations‐Stream Module
Author(s) -
Nguyen Huong Thi Thuy,
Kang Seong Ho
Publication year - 2020
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.11982
Subject(s) - resolution (logic) , total internal reflection fluorescence microscope , dna , superresolution , low resolution , computer science , content (measure theory) , physics , high resolution , computational biology , artificial intelligence , optics , image (mathematics) , biology , genetics , mathematics , remote sensing , geology , mathematical analysis , fluorescence
The DNA bp distance could be distinguished by the TIRF‐based SRRF‐stream module to create a super‐resolution image at subdiffraction limits. The resolution reached values up to ~100 nm, and objects can be detected at a minimum distance of 300 bp.