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Purity Assessment of Fumonisin B 1 by Quantitative 1 H NMR Spectroscopy
Author(s) -
Yun Suyeon,
Yoon Jeongbin,
Kim Byungjoo,
Ahn Sangdoo,
Choi Kihwan
Publication year - 2020
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.11981
Subject(s) - chemistry , fumonisin b1 , analytical chemistry (journal) , chromatography , nuclear magnetic resonance spectroscopy , mycotoxin , spectroscopy , absorbance , mass spectrometry , stereochemistry , physics , food science , quantum mechanics
Fumonisin B 1 (FB 1 ) a mycotoxin that contaminates grains and is related with liver and renal diseases. Although FB 1 levels in food are currently regulated, FB 1 's quantitation is complicated because of its lack of volatility and UV absorbance, preventing mass balance method from being employed for purity assessment of high‐purity standard. This study used quantitative nuclear magnetic resonance (qNMR) spectroscopy to assess the purity of FB 1 . Unlike mass balance method, qNMR is a universal and simple quantification method. Using benzoic acid as an internal standard, the purity of FB 1 was determined. The measurement parameters were optimized to obtain accurate results, and 1 H NMR peak homogeneity was confirmed by analyzing impurities separated using liquid chromatography. The purity of the FB 1 was 993 mg/g and expanded uncertainty ( k = 2.74) was 32 mg/g (3.2%). This result will be used to the development of the certified reference material for the FB 1 analysis in maize flour.