Premium
Benzoate Surfactants for Enhancing the Activity of Lipoprotein Lipase from Burkholderia Species in Organic Solvent
Author(s) -
Oh Yeonock
Publication year - 2019
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.11877
Subject(s) - chemistry , burkholderia , lipase , pulmonary surfactant , solvent , organic chemistry , organic solvent , enzyme , biochemistry , bacteria , chemical engineering , biology , genetics , engineering
Two benzoate surfactants were synthesized and examined as the additives for enhancing the activity of a lipoprotein lipase from Burkholderia species (BSLPL) in organic solvent. It was found that the benzoate surfactants enhanced the turnover number ( k cat ) by four orders of magnitude and the catalytic efficiency ( k cat / K m ) by three orders of magnitude. These results strongly suggest that the favorable interaction between the aromatic rings of surfactant tails and the hydrophobic residues around the active site of enzyme may help BSLPL maintain highly active open conformation in organic solvent.