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A Quencher‐Fluorophore‐Type Probe for Detection and Imaging of NADPH in Human Breast Cancer Cells
Author(s) -
Yu Geunhyeok,
Kim Sungryung,
Bae Se Won,
Yeo WoonSeok
Publication year - 2019
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.11826
Subject(s) - fluorophore , nicotinamide adenine dinucleotide phosphate , chemistry , nicotinamide adenine dinucleotide , fluorescence , intramolecular force , cofactor , dehydrogenase , human breast , photochemistry , biochemistry , cancer cell , stereochemistry , nad+ kinase , enzyme , cancer , biology , physics , quantum mechanics , oxidase test , genetics
A new fluorogenic trimethyl lock quinone (TLQ) derivative, designated as the quencher‐TLQ‐fluorophore‐type probe (Q‐TLQ‐F), was developed for selective detection of nicotinamide adenine dinucleotide phosphate (NADPH). By taking advantage of the well‐known facile intramolecular ring cyclization reaction (δ‐lactonization) of TLQ that can release a reporter molecule upon reduction, Q‐TLQ‐F was successfully applied for the detection of physiological NADPH generated by glucose‐6‐phosphate dehydrogenase in human breast cancer MDA‐MB‐231 cells.