Premium
Synthesis and Studies on Photophysical Properties of Rhodamine Derivatives for Bioimaging Applications
Author(s) -
Vanjare Balasaheb D.,
Mahajan Prasad G.,
Dige Nilam C.,
Phull Abdul Rehman,
Kim Song Ja,
Lee Ki Hwan
Publication year - 2019
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.11733
Subject(s) - solvatochromism , fluorescence , rhodamine , absorption (acoustics) , chemistry , proton nmr , photochemistry , solvent , rhodamine b , fluorescence spectroscopy , combinatorial chemistry , materials science , organic chemistry , physics , quantum mechanics , photocatalysis , composite material , catalysis
Rhodamine derivatives have been persistently used as fluorescent/solvatochromic dyes in various areas. For example, they are used as a labeling agent in biotechnology and a chemosensor. Also, rhodamine derivatives are used for cell imaging experiment. Herein, we report on the synthesis of fluorescent derivatives based on rhodamine B ( RHB1, RHB2, and RHB3 ). We confirmed the structures and purities of the synthesized probes i.e., RHB1, RHB2, and RHB3 by using analytical technique's viz . 1 H NMR, 13 C NMR, Mass and FT‐IR followed by exploring spectroscopic properties by using fluorescence/UV absorption spectroscopy. The effect of solvents on the fluorescence and absorption properties of the synthesized compounds has been comprehensively studied using a series of solvent. In addition, synthesized RHB1, RHB2 , and RHB3 were found to be non‐toxic against the cancer cell (MDA‐MB‐231). It is clear from the result of the conventional fluorescence microscopy that probe RHB2 exhibits significant optical properties as compared with those of RHB1 and RHB3 .