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Antimelanogenic Effects of Raphanus sativus L. var. niger Roots on α‐MSH Stimulated B16F10 Melanoma Cells
Author(s) -
Ko Ha Na,
Kim Jung Eun,
Jo Yeon Jeong,
Hong Seung Hyun,
Yang Da Wun,
Kim Gi Ok,
Lee Nam Ho
Publication year - 2018
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.11583
Subject(s) - raphanus , tyrosinase , chemistry , melanin , biochemistry , enzyme , botany , biology
This study was conducted to identify the antimelanogenic components from Raphanus sativus L. var. niger (black radish). Phytochemical study with the ethanol extract of black radish roots resulted in the isolation of six compounds: 3‐( E )‐(methylthio)methylene‐2‐pyrrolidinethione ( 1 ), ascorbigen ( 2 ), β‐sitosterol ( 3 ), palmitic acid ( 4 ), α‐linolenic acid ( 5 ), and 1‐linoleonyl glycerol ( 6 ). Among the isolates, the thione 1 efficiently inhibited melanin synthesis in a concentration‐dependent manner in α‐MSH stimulated B16F10 melanoma cells. Western blotting analysis for the key melanogenic enzymes showed that compound 1 decreased tyrosinase and tyrosinase‐related protein‐2 expressions. These results demonstrated that the black radish extract including pyrrolidinethione 1 could be useful as antimelanogenic agents in cosmetic or pharmaceutical applications.