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Real‐Time Monitoring of the Binding/Dissociation and Redox States of a Single Transition Metal Ions
Author(s) -
Jung SeungRyoung,
Lee SangWook,
Hohng Sungchul
Publication year - 2018
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.11443
Subject(s) - förster resonance energy transfer , cyanine , chemistry , metal ions in aqueous solution , ion , dissociation (chemistry) , fluorescence , photochemistry , redox , transition metal , metal , macromolecule , electron transfer , inorganic chemistry , organic chemistry , catalysis , biochemistry , physics , quantum mechanics
Fluorescence resonance energy transfer (FRET) between organic fluorophores is conventionally used to monitor binding/dissociation and conformational change of macromolecules. Here we use FRET between a cyanine dye and single transition metal ions (tmFRET) to monitor the binding/dissociation of transition metal ions to a synthetic polypeptide, and show that different transition metal ion species can be clearly distinguished based on their binding lifetimes and FRET efficiencies. Furthermore, we demonstrate that the redox state of cytochrome c can be successfully monitored at the single‐ion level using the tmFRET.