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Synthesis, Characterization, and Enhanced Cancer‐Imaging Application of Trans‐activator of Transcription Peptide‐conjugated Ultrasmall Gadolinium Oxide Nanoparticles
Author(s) -
Ahmad Mohammad Yaseen,
Cha Hyunsil,
Oh InTaek,
Tegafaw Tirusew,
Miao Xu,
Ho Son Long,
Marasini Shanti,
Ghazanfari Adibehalsadat,
Yue Huan,
Chae Kwon Seok,
Chang Yongmin,
Lee Gang Ho
Publication year - 2018
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.11404
Subject(s) - in vivo , gadolinium , conjugated system , magnetic resonance imaging , chemistry , in vitro , peptide , cancer cell , materials science , nuclear magnetic resonance , nuclear chemistry , cancer , medicine , biochemistry , organic chemistry , physics , microbiology and biotechnology , radiology , biology , polymer
We prepared gadolinium oxide (Gd 2 O 3 ) nanoparticles (GNPs) coated with a trans‐activator of transcription (TAT) peptide with cell‐penetrating ability ( i.e ., TAT‐GNPs) through one‐pot process. We characterized the particle diameter, surface‐coating structure, water proton relaxivities, and in vitro cellular toxicities of the TAT‐GNPs. We measured in vivo T 1 magnetic resonance images (MRI) in a model nude mouse with liver cancer prior and posterior to intravenous administration. The average particle diameter of the GNPs was 1.5 nm. The sample solution exhibited a longitudinal water proton relaxivity (r 1 ) of 18.2/s/mM (r 2 /r 1 = 1.6, r 2 = transverse water proton relaxivity), which is four to five times higher than those of commercial Gd‐chelates. The in vivo T 1 MRI exhibited positively (or T 1 ) enhanced contrasts in the mouse liver cancer after intravenous administration, demonstrating that the TAT‐GNPs acted as an enhanced cancer‐imaging agent similar to the cancer‐targeting agent in T 1 MRI.