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Photophysical Study of 2‐Fluorenyl‐1,2,3‐Triazole‐labeled 2′‐Deoxyuridine and Its Oligonucleotide
Author(s) -
Hong Seung Woo,
Hwang Gil Tae
Publication year - 2018
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.11349
Subject(s) - linker , fluorophore , fluorescence , chemistry , deoxyuridine , oligonucleotide , fluorene , nucleobase , triazole , photochemistry , click chemistry , uracil , chromophore , excimer , duplex (building) , dna , combinatorial chemistry , organic chemistry , biochemistry , physics , polymer , quantum mechanics , computer science , operating system
In this study, we prepared 2‐fluorenyl‐1,2,3‐triazol‐4‐yl‐2′‐deoxyuridine ( U FT ) and inserted it at the central position of an oligodeoxynucleotide (ODN) to investigate whether the triazole group is an efficient linker between fluorene and uracil. U FT displays different fluorescence intensities and emission maxima in different solvents, indicating its potential for application as an environmentally sensitive probe. Moreover, excimer emission from U FT was observed in water. However, U FT containing ODN did not exhibit selective thermal stability and fluorescence changes upon duplex formation with matched and single‐base mismatched targets. This highlighted the importance of selecting the appropriate linker to connect the fluorophore to the nucleobase when developing such DNA probes.