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Electrochemical Deposition of Protein‐conjugated Graphene by Pulse Reverse Technique
Author(s) -
Thirumalai Dinakaran,
Chang SeungCheol
Publication year - 2017
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.11309
Subject(s) - graphene , electrochemistry , conjugated system , electron transfer , electrode , biosensor , cytochrome c , oxide , glassy carbon , chemistry , deposition (geology) , materials science , inorganic chemistry , nanotechnology , photochemistry , organic chemistry , cyclic voltammetry , polymer , biochemistry , mitochondrion , paleontology , sediment , biology
A simple electrochemical deposition method for the immobilization of protein‐conjugated graphene onto an electrode has been developed. A unique one‐step electrochemical deposition of cytochrome c (Cyt c ) conjugated with electrochemically reduced graphene oxide ( ErGO ) onto the surface of glassy carbon electrode ( GCE ) without any cross‐linkers and the direct electron transfer between Cyt c and the electrode was obtained by the Cyt c – ErGO ‐modified GCE . The characteristics of the ErGO –Cyt c ‐modified GCE were compared with those of an ErGO ‐modified GCE without Cyt c . The direct electron transfer rate constant ( k et ) was found to be 6.2/s. This approach provides an avenue for the immobilization of various proteins and enzymes with ErGO and could be expanded for diverse biosensor applications.