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Quenching of Salicylaldehyde‐based Luminescence Probe via Dakin Reaction: Approach for Highly Selective Detection of Hydrogen Peroxide
Author(s) -
Tan NaiDi,
Yuan Yaqing,
Yin JianHang,
Xu Na
Publication year - 2017
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.11190
Subject(s) - luminescence , salicylaldehyde , chemiluminescence , detection limit , chemistry , quenching (fluorescence) , hydrogen peroxide , deprotonation , biocompatibility , photochemistry , analytical chemistry (journal) , nuclear chemistry , materials science , fluorescence , chromatography , stereochemistry , organic chemistry , optoelectronics , ion , schiff base , physics , quantum mechanics
A novel assay for H 2 O 2 based on luminescence quenching is developed from the Dakin reaction between deprotonated salicylaldehyde (D‐ SA ) and H 2 O 2 . Under alkaline condition, the phenolic hydroxyl group of SA is deprotonated, exhibiting good luminescence emission ability ( λ ex = 433 nm, λ em = 508 nm). Luminescence of D‐ SA is quenched by H 2 O 2 in a concentration‐dependent manner. Good linearity is obtained with a regression coefficient of 0.9989 in the range of 0.3 ~ 150 μM and a detection limit ( LOD ) is 30 nM . In addition, D‐ SA luminescence probe shows good biocompatibility and low cytotoxicity to HeLa cells through MTT assay. The feasibility for in vitro applications is demonstrated through confocal laser scanning microscopy.