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Enhancing Lysozyme Loading in Powderized Liposomes by Controlling Encapsulation Processes
Author(s) -
Ro Jieun,
Park Changhee,
Kim Jeong Tae,
Kim Hyeongmin,
Lee YoungEun,
Yoo SeungYup,
Hong SeongChul,
Lee Kyung Bin,
Lee Jaehwi
Publication year - 2017
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.11164
Subject(s) - lysozyme , liposome , phosphatidylcholine , chemistry , chromatography , particle size , chemical engineering , biophysics , biochemistry , membrane , biology , phospholipid , engineering
Liposomes have demonstrated great potentials as protein carriers in various pharmaceutical applications. However, low loading amount of proteins in liposomes have been a major challenge for maximizing the therapeutics potential of the proteins. We thus aimed to enhance the loading amount of a model protein, lysozyme, in liposomes by controlling key experimental variables of a reverse phase evaporation method. The loading amount of lysozyme in liposomes was evaluated with changing type of organic solvents used, weight ratio of lysozyme/phosphatidylcholine, and volume ratio of aqueous to organic phase along with particle characterization before and after freeze‐drying procedure. As a result, the loading amount of lysozyme in liposomes was considerably enhanced and the physical stability of the liposomes was maintained without any significant changes in the particle characteristics for 7 days. The findings of this study would be useful for highly efficient loading of protein therapeutics in liposomes, leading to improved therapeutic effects of the drugs.