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Synthesis of Tailed Metalloporphyrins Modified with 2‐Chloronicotinic Acid and Interactions with Human Serum Albumin
Author(s) -
Wang Shu Jun,
Peng Yu Ling,
Zhang Cheng Gen,
Ma Qi Peng,
Peng Xiao Xia,
Ren Li Lei
Publication year - 2017
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.11163
Subject(s) - quenching (fluorescence) , chemistry , fluorescence , human serum albumin , binding constant , proton nmr , fluorescence spectroscopy , hydrophobic effect , analytical chemistry (journal) , organic chemistry , binding site , chromatography , biochemistry , physics , quantum mechanics
Two kinds of free‐base tailed porphyrins modified with 2‐chloronicotinic acid and the corresponding Zn porphyrins have been synthesized. They have been characterized by elemental analysis and NMR , UV /Vis, fluorescence spectra, and infrared spectroscopies. Their configurations have been optimized through theoretical calculations. The fluorescence quantum yields were determined by a comparative method. The interactions between the Zn porphyrins and human serum albumin have been studied by means of fluorescence spectra. The experimental results showed that the interaction mechanism involved a combined fluorescence quenching process (static and dynamic quenching) and that the main driving force was hydrophobic in nature. Quenching constants, binding constants, thermodynamic parameters, and binding distances have been determined.