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Anti‐Inflammatory Constituents from the Branches of Pourthiaea villosa (Thunb.) Decne
Author(s) -
Ko Ha Na,
Kang Ji Mi,
Kim Hyung Min,
Han JongHeon,
Lee Nam Ho
Publication year - 2017
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.11158
Subject(s) - chemistry , daucosterol , nitric oxide , gallic acid , ethyl acetate , phytochemical , anti inflammatory , traditional medicine , ursolic acid , nitric oxide synthase , quercitrin , biochemistry , pharmacology , chromatography , rutin , organic chemistry , biology , chemical constituents , medicine , antioxidant
In this study, the extract from Pourthiaea villosa Decne branches was investigated and their anti‐inflammatory constituents were identified. The prepared ethanol extract was fractionated successively to afford n ‐hexane, ethyl acetate ( EtOAc ), n ‐butanol, and aqueous layers. In the examination of the anti‐inflammatory activities, EtOAc layer was observed to have good nitric oxide production inhibitory property in lipopolysaccharide ( LPS )‐stimulated RAW 264.7 cells. Phytochemical studies for EtOAc layer led to isolation of eight constituents such as ursolic acid ( 1 ), arjunolic acid ( 2 ), daucosterol ( 3 ), myricanol ( 4 ), gallic acid ( 5 ), myricetin ( 6 ), myricitrin ( 7 ), and quercitrin ( 8 ). The isolate 4 efficiently inhibited nitric oxide production in a dose‐dependent manner in LPS ‐induced RAW 264.7 cells. The production of inducible nitric oxide synthase ( iNOS ) enzyme protein was decreased by compound 4 , which showed that the observed NO inhibition could be interconnected to down‐expression of iNOS protein. In addition, compound 4 reduced the production of TNF ‐α, IL ‐1β, and IL ‐6 in a concentration‐dependent manner. This study demonstrated that the P. villosa extract containing myricanol ( 4 ) could be considered potential anti‐inflammatory agents in pharmaceutical or cosmetic industries.