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Toxicological Assessment of Microcystin‐LR to Zebrafish ( Danio rerio ) Using Metabolomics
Author(s) -
Yoon Dahye,
Choi Hyeonsoo,
Noh Hyeonkyung,
Ok Young Jun,
Oh Sangah,
Ma Seohee,
Yoon Changshin,
Kim Suhkmann
Publication year - 2017
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.11112
Subject(s) - danio , zebrafish , betaine , taurine , glutathione , metabolomics , chemistry , metabolic pathway , biochemistry , detoxification (alternative medicine) , creatine , biology , metabolism , enzyme , chromatography , amino acid , medicine , gene , alternative medicine , pathology
Microcystins are one of the most frequently found hepatotoxic cyclic peptides in fresh‐ and brackish‐water. Zebrafish ( Danio rerio ) were exposed to 20 ppb of microcystin‐LR (MCLR) for 72 h in order to investigate the metabolic responses. Proton nuclear magnetic resonance ( 1 H‐NMR) spectroscopy was used to analyze the metabolic profiles. As a result, male and female zebrafish showed different patterns of metabolic perturbation in relation to their detoxification profiles. Glutathione was reduced for the MCLR excretion, and fumarate and creatine were increased for the energy demands required for detoxification in the male zebrafish. The correlation of metabolites in the metabolic pathway network was changed due to MCLR exposure. The correlations connected with myo‐inositol, betaine, and NADPH considerably altered in the MCLR‐exposed group.