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Isolation and Quantification of Phenolic Compounds in Acer tegmentosum by High Performance Liquid Chromatography
Author(s) -
Lee JungIn,
Hwang In Hyun,
Jang Tae Su,
Na MinKyun
Publication year - 2017
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.11099
Subject(s) - tyrosol , scopoletin , chemistry , chromatography , high performance liquid chromatography , salidroside , polyphenol , rutin , catechin , phenols , antioxidant , organic chemistry , medicine , alternative medicine , pathology
Determination of bioactive chemicals in Acer tegmentosum is essential for its industrial application. Twelve phenolic compounds were isolated from A. tegmentosum . Specific seven compounds in the plant were simultaneously analyzed by high performance liquid chromatography‐ UV . The content of bioactive chemicals in the branch extract was determined to be 3.38 mg/g of (−)‐epigallocatechin, 66.95 mg/g of salidroside, 39.61 mg/g of tyrosol, 17.04 mg/g of catechin, 7.39 mg/g of 1,2,4‐tri‐ O ‐galloyl‐β‐ d ‐glucose, 8.88 mg/g of 6‐ O ‐galloylsalidroside, and 4.61 mg/g of scopoletin. The method was validated with linearity ( R 2 > 0.9947), as well as intra‐ and inter‐day precision (0.07–2.77%) and accuracy (>97.0%). This analytical method is expected to be applied for standardization of dietary food containing A. tegmentosum .