Isolation and Quantification of Phenolic Compounds in Acer tegmentosum by High Performance Liquid Chromatography

Determination of bioactive chemicals in Acer tegmentosum is essential for its industrial application. Twelve phenolic compounds were isolated from A. tegmentosum . Specific seven compounds in the plant were simultaneously analyzed by high performance liquid chromatography‐ UV . The content of bioactive chemicals in the branch extract was determined to be 3.38 mg/g of (−)‐epigallocatechin, 66.95 mg/g of salidroside, 39.61 mg/g of tyrosol, 17.04 mg/g of catechin, 7.39 mg/g of 1,2,4‐tri‐ O ‐galloyl‐β‐ d ‐glucose, 8.88 mg/g of 6‐ O ‐galloylsalidroside, and 4.61 mg/g of scopoletin. The method was validated with linearity ( R 2 > 0.9947), as well as intra‐ and inter‐day precision (0.07–2.77%) and accuracy (>97.0%). This analytical method is expected to be applied for standardization of dietary food containing A. tegmentosum .