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In Situ Growth of Plasmonic Gold Nanoparticles for the Direct and Sensitive Colorimetric Assay of Glucose
Author(s) -
Weng Shaohuang,
Feng Feng,
Guo Honghui,
Wu Namei,
Zheng Yanjie,
Lin Zhen,
Lin Xinhua
Publication year - 2017
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.11095
Subject(s) - absorbance , colloidal gold , chemistry , detection limit , in situ , colorimetry , nanoparticle , plasmonic nanoparticles , chromatography , naked eye , nuclear chemistry , nanotechnology , materials science , organic chemistry
Gold nanoparticles (Au NPs )‐based colorimetric assay for the highly sensitive and selective detection of glucose has been conducted. The sensing, which was based on the in situ growth of Au NPs in the presence of glucose, was developed with the solution changing from colorless to red. This change could be observed with the naked eye or a ultraviolet–visible ( UV –vis) spectrophotometer. Moreover, adding trace amounts of Tween‐20 kept Au NPs well spaced. Under the optimal assay conditions, the absorbance of Au NPs at 550 nm was proportional to glucose concentration within 400–1800 μM with a linear regression equation, and the detection limit was 6.28 μM . Some common interfering compounds in beverages, including other sugars, food additives, and amino acids, did not affect glucose sensing. Therefore, the proposed Au NP ‐based colorimetric assay could be successfully used to determine glucose in beverages.