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Identification of Mitotic Blockers by Phenotypic Screening of a Clinical Compound Library
Author(s) -
Son You Hwa,
Park Chi Hoon,
Choi Sang Un
Publication year - 2016
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.10999
Subject(s) - hela , mitosis , cytotoxicity , cell cycle , cytotoxic t cell , chemistry , cell , cancer research , pharmacology , biology , in vitro , microbiology and biotechnology , biochemistry
A phenotypic assay was performed using a clinical compound library to identify the new anticancer agents. HeLa cells were treated with the clinical compounds for 20 h, and then screened for cell morphology to check the mitotic arrest. A total of 2320 clinical compounds were tested, of which two anthelmintic compounds, oxibendazole and oxfendazole, were found to induce mitotic arrest in HeLa cells. Additionally, cell‐cycle analysis revealed strong G2 /M arrest by these anthelmintics. Moreover, the two compounds, especially oxibendazole, exhibited highly cytotoxic effects on several cancer cell lines. In conclusion, we suggest repositioning of the two anthelmintics, oxibendazole and oxfendazole, if normal cells are not affected by these compounds, to cancer treatment.