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Multiplex Human Immunodeficiency Virus 1/2 and Hepatitis C Virus Antibodies Simultaneous Detection Using Three‐Dimensional Sol–Gel Nanoporous Capturing Technology
Author(s) -
Hwang Kyung Hoon,
Ki Yeona,
Lee Young Mi,
Lee Jin Ah,
Choi In Kyoung,
Park Jun Sung,
Kim Jinhong,
Kang Jasmine,
Kukushkin Yury,
Oh Eun Jee,
Han Kyung Ja,
Kim Soyoun
Publication year - 2016
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.10969
Subject(s) - multiplex , hepatitis c virus , virology , immunoassay , antibody , human immunodeficiency virus (hiv) , virus , multiplex polymerase chain reaction , hepatitis b virus , biology , immunology , polymerase chain reaction , bioinformatics , biochemistry , gene
The sol–gel nanoporous capturing technology represents a powerful approach where the sol–gel matrix constrains the motion of the encapsulated biomolecules (proteins, peptides, chemicals, antibodies, nucleotides, etc.) without physical adsorption or any modifications. This technology can be applied to multiplex immunoassay platform because several disease markers can be immobilized and tested at once. In this study, we have tested several thousand specimens using Hi3‐1 Multiplex human immunodeficiency virus ( HIV ) 1/2 and hepatitis C virus ( HCV ) antibody detection kit at two clinical trials institutes. These results showed a highest sensitivity (100%, n = 500 HIV ‐positive specimens and n = 400 HCV ‐positive) and specificity (99.99% for HIV and 99.83% for HCV , n = 7706 negative specimens) by using Hi3‐1 kit, which screens HIV1 /2 and HCV antibodies simultaneously. Finally, we suggested that our screening technology was successfully utilized as a multiplex HIV and HCV diagnostic tool for blood bank screening.