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Identification of Anthocyanins in Berry Species Using LC‐ESI‐MS Combined with High‐Speed Counter‐Current Chromatography
Author(s) -
Jin Young Ho,
Shin Hyun Ju,
Ahn Hye Jung,
Jang Bong Keun,
Park Na Hyun,
Hong Jongki
Publication year - 2016
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.10915
Subject(s) - berry , chemistry , glycoside , flavonols , chromatography , flavonoid , stereochemistry , organic chemistry , botany , antioxidant , biology
Anthocyanins ( ACs ) and flavonoids were isolated from three different berry species (blueberry, black chokeberry, and cranberry) via high‐speed counter‐current chromatography ( HSCCC ). A total of 19 ACs and 9 flavonoids in three berry extracts were characterized using LC‐ESI‐MS / MS . The MS / MS spectra of ACs and flavonoids produced a commonly abundant [M‐gluc] + ion due to the easy cleavage of the glycosidic ether bond, which is directly reflected the structural information of the aglycon moiety and linked glucose. Among the identified 19 ACs , the major compounds were identified as glycosylated ACs linked with 3‐ O ‐galactose, 3‐ O ‐glucose, 3‐ O ‐arabinoside, and 3‐ O ‐xylose. Three ACs and four flavonoids were the first time observed in three berry extracts. Particularly for the black chokeberry extract, the total amounts of ACs were 10‐fold higher than other two berry extracts. LC‐MS / MS combined with HSCCC will be alternative technique for identifying new ACs and for the quality evaluation of ACs in colored fruits.