Premium
Metal‐doped Nanoparticles for Detection of Carbohydrate Antigen 15‐3 in Human Serum Using a Sandwich‐Type ICP‐MS Immunoassay
Author(s) -
Jang Hana,
Lim H. B.
Publication year - 2016
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.10884
Subject(s) - immunoassay , detection limit , inductively coupled plasma mass spectrometry , chromatography , chemistry , nanoparticle , mass spectrometry , inductively coupled plasma , analytical chemistry (journal) , materials science , nanotechnology , antibody , plasma , physics , quantum mechanics , immunology , biology
This work presents a method to determine carbohydrate antigen 15‐3 ( CA15 ‐3) in serum using sandwich‐type inductively coupled plasma-mass spectrometry immunoassay. For this, Cd, Cs‐doped magnetic nanoparticles ( MNPs ) for target extraction and ratiometric measurement to enhance the calibration linearity were synthesized by co‐precipitation, of which the size was in the size range of 21–24 nm. The numbers of doped Cd and Cs atoms were in the range of 2.5 × 10 3 to 3.5 × 10 3 per MNP . The new synthetic method simplifies the synthetic procedure significantly with high flexibility for doping. Rare earth metal‐doped silica NPs ( SNPs ), i.e. , Gd, Eu, or Ce, were also synthesized for detection through metal‐chelation and microemulsion method, with a final size range of 70–85 nm. For application, carbohydrate antigen 15‐3 ( CA15 ‐3) was spiked into a human serum and determined by the sandwich‐type ICP‐MS immunoassay using the synthesized nanoparticles after blank subtraction. The obtained limit of detection ( LoD ) without any sample treatment was 2.70 × 10 −4 U/ mL , which was about 2.28 × 10 4 times better than that of enzyme‐linked immunosorbent assay ( ELISA ).