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Graphene Oxide‐mediated Fluorescence Quenching of Green Fluorescent Protein for Biomedical Applications
Author(s) -
Tran LanHuong,
Lee ChangWoo,
Kang Tai Jong,
Jang SeiHeon
Publication year - 2016
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.10850
Subject(s) - fluorescence , quenching (fluorescence) , graphene , covalent bond , photochemistry , green fluorescent protein , absorption (acoustics) , chemistry , oxide , fluorescence in the life sciences , absorption spectroscopy , materials science , förster resonance energy transfer , nanotechnology , organic chemistry , optics , biochemistry , physics , composite material , gene
We investigated the fluorescence property of green fluorescent protein ( GFP ) with graphene oxide ( GO ) for both non‐covalent and covalent interactions. The mechanism of GO ‐mediated quenching of GFP fluorescence is the combination of both static and dynamic quenching. Although GFP exhibits two absorption bands with similar fluorescence emission spectra, the quenching efficiency of the two absorption bands from non‐covalent immobilization were different—one with absorption at 460 nm, showing a higher fluorescence resonance transfer efficiency than the other absorption at 395 nm. We determined the apparent Stern–Volmer quenching constant ( K app ) of GFP for GO to be 2.6 × 10 −7 mL/g. However, GFP fluorescence disappeared upon covalent immobilization on GO .