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Preparation of Homogeneous Solid Samples for Reproducible and Quantitative MALDI
Author(s) -
Ahn Sung Hee,
Park Kyung Man,
Moon Jeong Hee,
Lee Seong Hoon,
Kim Myung Soo
Publication year - 2016
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.10704
Subject(s) - analyte , chemistry , homogeneous , sample preparation , desorption , chromatography , analytical chemistry (journal) , calibration curve , matrix (chemical analysis) , matrix assisted laser desorption/ionization , homogeneity (statistics) , mass spectrometry , detection limit , organic chemistry , physics , statistics , mathematics , adsorption , thermodynamics
Preparing samples with good homogeneity is one of the requirements for generating reproducible matrix‐assisted laser desorption ionization ( MALDI ) spectra and for using them in analyte quantification. Thus far, we have not been successful in producing homogeneous solid samples from popular matrixes apart from α‐cyano‐4‐hydroxycinnamic acid ( CHCA ). In this work, we demonstrate the production of an outwardly homogeneous solid sample by loading a methanol solution of a matrix into a shallow reservoir on a coated MALDI sample plate and then vacuum‐drying it. Out of ten popular matrixes tested, seven yielded homogeneous samples. These were 9‐aminoacridine, 6‐aza‐2‐thiothymine, CHCA , 2,5‐dihydroxybenzoic acid, ferulic acid, sinapinic acid, and 2,4,6‐trihydroxyacetophenone. For MALDI with these matrixes, linear calibration curves covering wide dynamic ranges were acquired. Performances of these matrixes in various aspects of analyte quantification have been investigated.