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Microchip Electrophoresis with Enhanced Dark‐Field Illumination Detection for Fast Separation of Native Single Super‐Paramagnetic Nanoparticles
Author(s) -
Zhang Peng,
Park Sangyoon,
Kang Seong Ho
Publication year - 2015
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.10219
Subject(s) - electric field , nanoparticle , electrophoresis , dissociation (chemistry) , analytical chemistry (journal) , materials science , zeta potential , paramagnetism , chemistry , nuclear magnetic resonance , chromatography , nanotechnology , physics , condensed matter physics , quantum mechanics
Fast microchip electrophoretic ( ME ) separation of native super‐paramagnetic nanoparticles ( SPMNPs ) with different functional groups ( i.e ., NH 2 , OH , and COOH ) was investigated at the single‐particle level using enhanced dark‐field illumination detection with high signal‐to‐noise ratio. The dynamics provide evidence of the random motion of individual native nanoparticles and their real‐time velocities within a microchip with or without an electric field. The SPMNPs were introduced into the microchip by magnetic force, and separation was performed at different electric field strengths; neither low (≤30 V/cm) nor high (≥70 V/cm) applied electric field strengths provided separation above baseline (resolution > 1.5) due to the random motion of the nanoparticles. Based on the optimized separation conditions obtained at the single‐particle level, the individual SPMNPs were separated successfully from the model nanoparticle mixture within ~75 s using differences in acid dissociation constants and zeta potentials.