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Longitudinal Assessment of Peptide Recoveries from a Sample Solution in an Autosampler Vial for Proteomics
Author(s) -
Cho Ha Ra,
Park Jun Seo,
Wood Troy D.,
Choi Yong Seok
Publication year - 2015
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.10082
Subject(s) - vial , chromatography , peptide , chemistry , formic acid , aqueous solution , mass spectrometry , organic chemistry , biochemistry
Relatively few studies on time‐related peptide losses have been reported and previous studies on the differential peptide recovery from a sample solution over a period had limitations. Thus, here, longitudinal peptide losses during sample solution storage in an autosampler vial over 145 h were assessed using nanoliquid chromatography and tandem mass spectrometry. In this study, hydrophilic peptide losses from a solution stored in a vial were observed regardless of materials of vials and the possibility of hydrophobic peptide losses brought by reasons other than hydrophobic peptide adsorption onto vials was found. Additionally, the preparation of a proteomics sample using 0.1% aqueous formic acid solution (0.1% FA ) and a polypropylene vial, 0.1% FA and a glass vial, or the aqueous mixture of 5 M urea, 1 M thiourea, and 0.1% FA and a polymethylpentene vial proved to produce the most reliable longitudinal recovery of peptides from a solution stored in a vial.