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High‐rate ethanol production at low pH using the anaerobic granular sludge process
Author(s) -
Tamis Jelmer,
Joosse Bart,
Leeuw Kasper,
Kleerebezem Robbert
Publication year - 2021
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.27708
Subject(s) - fermentation , bioprocess , chemistry , food science , chemical oxygen demand , chromatography , biochemistry , wastewater , chemical engineering , waste management , engineering
In this study, we investigated the operational performance and product spectrum of glucose‐fermenting anaerobic granular sludge reactor at pH 4. A selective environment for the growth of granules was implemented by the introduction of a 2 min settling phase, a hydraulic retention time of 6 h and a solid retention time of 12 ± 3 days. The fermentation products were ethanol, lactate, and volatile fatty acids (VFA) with yields of 0.55 ± 0.03, 0.15 ± 0.02, and 0.20 ± 0.04 gram chemical oxygen demand (gCOD)/gCOD glucose, respectively. The obtained product spectrum was remarkably different from the VFA‐dominated product spectrum reported in a previous study when the same system was operated at higher pH (4.5–5.5). The shift in product spectrum coincided with a shift in the microbial community structure with the dominance of eukaryotic Candida tropicalis, Pichia jaroonii , and prokaryotic Lactobacillus species instead of the Clostridia species obtained at higher pH‐values. The control of the microbiomes and the associated product spectra provides bioprocess engineers with the option to tailor a suitable precursor compound mixture for subsequent chain elongation fermentation or PHA biopolymer production.